Nein is a village in Israel.

Nein may also refer to: The Nein, an American indie rock band The Nein EP, their 2004 recording Nein, the Japanese band Sound Horizon's 8th major album "Nein" (song), a song by King Gizzard & the Lizard Wizard Nein, the German word for "no"

Persons with the surname Henry Nein (1860-1933), an American politician Jo Nein, pseudonym used by Norwegian writer Nini Roll Anker (1873–1942) Scott Nein (in office 1991-2004), an American politician Timothy Nein (in action in 2005), a United States soldier, recipient of Distinguished Service Cross