Mathlouthi () is a Tunisian surname which is – without the Arabic definite article Al-/El- – also common among the Tunisians in France. Notable people with this family name include:

Ahmed Mathlouthi (born 1989), Tunisian swimmer Ali Mathlouthi (born 1987), French-Tunisian footballer Aymen Mathlouthi (born 1984), Tunisian footballer Emel Mathlouthi (born 1982), Tunisian singer-songwriter Hamza Mathlouthi (born 1992), Tunisian footballer Maroua Mathlouthi (born 1988), Tunisian swimmer

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