The 2023–24 Coupe de France preliminary rounds, Nouvelle-Aquitaine is the qualifying competition to decide which teams from the leagues of the Nouvelle-Aquitaine region of France take part in the main competition from the seventh round.

A total of fourteen teams will qualify from the Nouvelle-Aquitaine section of the 2023–24 Coupe de France preliminary rounds.

In 2022–23, four teams from the region progressed to the 8th round, but all were eliminated at that stage. Fourth tier Bergerac Périgord FC and Trélissac-Antonne Périgord FC were defeated by Ligue 2 sides Niort and Bordeaux respectively; fifth tier US Lège Cap Ferret lost to third tier LB Châteauroux and fifth tier SO Châtellerault lost to Avoine OCC from the same division.

Draws and fixtures On 27 July 2023, the league announced that 700 teams from the region had entered the competition. 558 teams took part in the first round, being all the teams registered from the Departmental level divisions, and all but 35 Régional 3 teams. The exempted teams were chosen based on their performance in the previous seasons competition. The second round draw saw all 125 remaining teams from the regional divisions enter the competition. The third round draw was published on 7 September 2023, with the 10 teams from Championnat National 3 entering at this stage.

The fourth round draw, including the four teams from Championnat National 2, was carried out live on the Facebook page of the league on 25 September 2023.

The fifth round draw, which included 1 team from Championnat National, took place on 9 October 2023. The sixth and final regional round was drawn on 19 October 2023.

First round These matches were played on 26 and 27 August 2023.